Journal: Cell Death & Disease

Article Title: MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4

doi: 10.1038/s41419-020-02789-z

Figure Lengend Snippet: a Expression level of miR-3622a-3p was detected in CRC cell lines and NCM460 cell line by qRT-PCR. b Expression of miR-3622a-3p was increased in DLD-1 by miR-3622a-3p mimics transfection. c MiR-3622a-3p expression level was reduced in HCT116 by miR-3622a-3p inhibitor transfection. d , e The effect of miR-3622a-3p on proliferation of CRC cells was evaluated by CCK-8 cell proliferation assay. f , g Colony forming ability of CRC cells was negatively corelated with miR-3622a-3p expression level. h , i The results of EDU assay suggested overexpression of miR-3622a-3p suppressed proliferation of CRC cells while knockdown of miR-3622a-3p promoted CRC cell proliferation. All data are from three independent experiments and are presented as the means ± SD (* p < 0.05, ** p < 0.01).

Article Snippet: Human normal colon epithelial cell line NCM460 was obtained from American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Quantitative RT-PCR, Transfection, CCK-8 Assay, Proliferation Assay, EdU Assay, Over Expression, Knockdown

Journal: Journal of Gastrointestinal Oncology

Article Title: The roles and mechanisms of APOL1 in the development of colorectal cancer

doi: 10.21037/jgo-24-275

Figure Lengend Snippet: Effect of APOL1 on the biological behavior of CRC cells. (A) qRT-PCR was used to determine the APOL1 mRNA levels in HCT116, SW1116, and NCM460 cells. (B) In HCT116 cells, qRT-PCR was used to determine the interference efficacy of shAPOL1#1/2/3. (C) CCK8 assay was used to evaluate the proliferative capacity of HCT116 and SW1116 cells. (D) The count of clones in HCT116 and SW1116 cells was determined using the colony formation test. Staining method: crystal violet. Magnification: ×100. (E) Wound healing test was used to assess the migratory capacity of HCT116 and SW1116 cells (magnification: ×40). (F) The Transwell test was used to determine HCT116 and SW1116 cells’ invasion ability (magnification: ×100). Staining method: crystal violet. Each group, n=3. *, P<0.05 vs. NCM460 group; **, P<0.01; ***, P<0.001 vs . sh-NC group. sh-NC, shRNA-negative control; sh-APOL1, shRNA-apolipoprotein L1; OD, optical density; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK8, Cell Counting Kit 8.

Article Snippet: Shanghai iCell Bioscience Inc. (Shanghai, China) provided the human normal colon epithelial cells NCM460 (iCell-h373) and CRC cell lines [HCT116 (iCell-h071) and SW1116 cells (iCell-h201)].

Techniques: Quantitative RT-PCR, CCK-8 Assay, Clone Assay, Staining, shRNA, Negative Control, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Apoptosis

Article Title: SREBP2 confers ferroptosis resistance by targeting GPX4 in colorectal cancer

doi: 10.1007/s10495-025-02209-7

Figure Lengend Snippet: SREBP2 was upregulated in human colorectal cancer. A . Real-time PCR analysis of SREBP2 expression levels in colorectal cancer and adjacent tissues. B , C . Western blotting analysis of SREBP2 in CRC tissues and adjacent tissues. The quantitative results of SREBP2 expression levels were evaluated. D , E . Immunohistochemistry of SREBP2 in tumor sections generated from CRC samples, utilizing adjacent tissues as a control. Scale bar, 50 μm. Analysis of SREBP2 IHC scores in two groups of tissue samples. F . Expression levels of SREBP2 in different CRC cell lines and normal NCM460 cells were determined by real-time PCR. G , H . Western blotting analysis of SREBP2 in different CRC cell lines and normal NCM460 cells. The quantitative results of SREBP2 expression levels were evaluated. The data are presented as the mean ± SEM of at least three independent experiments. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The human colon epithelial cell line NCM460 and six colorectal cancer cell lines—SW480, RKO, Lovo, HT29, HCT116 and Caco2—were obtained from Procell Life Science & Technology Co (Wuhan, China).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunohistochemistry, Generated, Control